Protoplast isolation technical and teaching notes

Received Aug 22; Accepted Sep This article has been cited by other articles in PMC.

Protoplast isolation technical and teaching notes

Semester 1 SMBB This course encompasses the basic principles and techniques involved in molecular biology which will enable students to apply these techniques in the genetic engineering laboratory. The introductory lecture will expose students to genetic engineering and its application in various sectors of the industry such as agriculture, medical, pharmaceutical, environmental, etc.

The following lectures will revolve around techniques in genetic engineering, cloning of heterologous genes in the Escherichia coli host which will include common procedures in molecular biology, enzymes important in molecular biology, plasmids and bacteriophage as cloning vectors, gene libraries preparation and screening for gene of interest.

Molecular Sieve Chromatography Kit (Teaching) Part E Notes: Kit contents: DNA-RNA-Nucleotide Mix - Column - Buffer Customers Also Viewed: Plasmid Isolation Kit from Bacterial cells (Teaching) Protoplast Fusion Kit from Plants (Teaching) Genomic DNA Extraction (from leaves) Kit (Teaching). Chart and Diagram Slides for PowerPoint - Beautifully designed chart and diagram s for PowerPoint with visually stunning graphics and animation effects. Our new CrystalGraphics Chart and Diagram Slides for PowerPoint is a collection of over impressively designed data-driven chart and editable diagram s guaranteed to impress any audience. Protoplast isolation and culture of a brown alga, Undaria pinnatifida Translate with. translator. This translation tool is powered by Google. FAO is not responsible for the accuracy of translations. Technical Cooperation; Offices. Regional Office for Africa; Regional Office for Asia and the Pacific;.

Before semester ends, students are to produce a group poster relevant to genetic engineering as a form of teamworking experience. Peer group evaluation is mandatory. Some of the concepts taught will be applied in this laboratory practical.

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Students will have the opportunity to hands on the isolation of genomic DNA, agarose gel electrophoresis, PCR amplification, DNA ligation, preparation of competent cells, transformation, and lastly verify the clones of interest by plasmid DNA extraction and DNA restriction digestion.Suspension culture, Protoplast isolation, culture and fusion.

Clonal propagation: Shoot-tip and axillary bud culture. Techniques of cell and tissue culture: Preparation of explant materials, initiation of cultures, micropropagation through organogensis and embryogensis, artificial seeds.

Preparation of recombinant polysaccharide-degrading enzymes from the marine bacterium, Pseudomonas sp.

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Protoplast isolation technical and teaching notes

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Protoplast isolation technical and teaching notes

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